产品描述:Rabbit polyclonal antibody to PHF17免疫原:Recombinant full length protein of human PHF17纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)基因名称:JADE1相关名称:KIAA1807; PHF17; Protein Jade-1; Jade family PHD finger protein 1; PHD finger protein 17
基因编号(人):
79960;
基因编号(小鼠):
269424;
蛋白编号(人):
Q6IE81;
蛋白编号(小鼠):
Q6ZPI0;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PHF17 expression in mouse kidney (A), mouse liver (B) whole cell lysates. (Predicted band size: 95 kD; Observed band size: 95 kD)
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Immunofluorescent analysis of PHF17 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.