产品描述：Rabbit polyclonal antibody to IRSp53免疫原：Recombinant full length protein of human IRSp53纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：BAIAP2相关名称：Brain-specific angiogenesis inhibitor 1-associated protein 2; BAI-associated protein 2; BAI1-associated protein 2; Protein BAP2; Fas ligand-associated factor 3; FLAF3; Insulin receptor substrate p53/p58; IRS-58; IRSp53/58; Insulin receptor substrate protein of 53 kDa; IRSp53; Insulin receptor substrate p53 基因编号(人)： 10458; 基因编号(小鼠)： 108100; 基因编号(大鼠)： 117542; 蛋白编号(人)： Q9UQB8; 蛋白编号(小鼠)： Q8BKX1; 蛋白编号(大鼠)： Q6GMN2; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IRSp53 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 56; 57; 59; 60 kD; Observed band size: 53; 58 kD)
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  Immunofluorescent analysis of IRSp53 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.