产品描述：Rabbit polyclonal antibody to RhoC免疫原：Recombinant full length protein of human RhoC纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)基因名称：RHOC相关名称：ARH9; ARHC; Rho-related GTP-binding protein RhoC; Rho cDNA clone 9; h9 基因编号(人)： 389; 基因编号(小鼠)： 11853; 蛋白编号(人)： P08134; 蛋白编号(小鼠)： Q62159; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RhoC expression in Jurkat (A), A549 (B), PC12 (C) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD)
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  Immunofluorescent analysis of RhoC staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.