产品描述：Rabbit polyclonal antibody to EEF1A1免疫原：Recombinant full length protein of human EEF1A1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)基因名称：EEF1A1相关名称：EEF1A; EF1A; LENG7; Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7 基因编号(人)： 1915; 基因编号(小鼠)： 13627; 基因编号(大鼠)： 171361; 蛋白编号(人)： P68104; 蛋白编号(小鼠)： P10126; 蛋白编号(大鼠)： P62630; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EEF1A1 expression in MCF7 (A), Jurkat (B), HepG2 (C), SHSY5Y (D) whole cell lysates. (Predicted band size: 47; 50 kD; Observed band size: 50 kD)
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  Immunofluorescent analysis of EEF1A1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.