说明书
MSDS产品描述：Mouse monoclonal antibody to Peroxiredoxin 1免疫原：Recombinant protein corresponding to human Peroxiredoxin 1.纯化方式：Affinity chromatography克隆类型：Monoclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)基因名称：PRDX1相关名称：PAGA; PAGB; TDPX2; Peroxiredoxin-1; Natural killer cell-enhancing factor A; NKEF-A; Proliferation-associated gene protein; PAG; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2
基因编号(人)：
5052;
基因编号(小鼠)：
18477;
基因编号(大鼠)：
117254;
蛋白编号(人)：
Q06830;
蛋白编号(小鼠)：
P35700;
蛋白编号(大鼠)：
Q63716;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Peroxiredoxin 1 expression in MCF7 (A), mouse brain (B), rat kidney (C) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 21 kD) -
Immunofluorescent analysis of Peroxiredoxin 1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).