产品描述:Mouse monoclonal antibody to GRP78免疫原:KLH-conjugated synthetic peptide encompassing a sequence of human GRP78. The exact sequence is proprietary.纯化方式:Affinity chromatography克隆类型:Monoclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/1000 - 1/2000), IH (1/100 - 1/200)基因名称:HSPA5相关名称:GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
基因编号(人):
3309;
蛋白编号(人):
P11021;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GRP78 expression in Hela (A), rat liver (B) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 78 kD)
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Immunohistochemical analysis of GRP78 staining in human colon cancer,human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.