产品描述：Mouse monoclonal antibody to CD54免疫原：Native purified human CD54.纯化方式：The antibody was purified by affinity chromatography.克隆类型：Monoclonal (clone: 15.2)产品形式：Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.稀释比：IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)基因名称：ICAM1相关名称：Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54 基因编号(人)： 3383; 蛋白编号(人)： P05362; 使用指导：1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD54 Antibody, followed by anti-mouse IgG PE.