产品描述:Rabbit polyclonal antibody to Collagen 17 alpha 1免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 17 alpha 1. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/2000), IH (1/50 - 1/200)基因名称:COL17A1相关名称:BP180; BPAG2; Collagen alpha-1(XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2
基因编号(人):
1308;
基因编号(小鼠):
12821;
蛋白编号(人):
Q9UMD9;
蛋白编号(小鼠):
Q07563;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Collagen 17 alpha 1 expression in rat kidney (A), HCT116 (B), HepG2 (C), 3T3L1 (D) whole cell lysates. (Predicted band size: 150 kD; Observed band size: 130 kD)
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Immunohistochemical analysis of Collagen 17 alpha 1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.