说明书
MSDS产品描述：Rabbit polyclonal antibody to GPR66免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR66. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：NMUR1相关名称：GPR66; Neuromedin-U receptor 1; NMU-R1; G-protein coupled receptor 66; G-protein coupled receptor FM-3
基因编号(人)：
10316;
基因编号(小鼠)：
14767;
基因编号(大鼠)：
65276;
蛋白编号(人)：
Q9HB89;
蛋白编号(小鼠)：
O55040;
蛋白编号(大鼠)：
Q9JJI5;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GPR66 expression in rat testis (A), mouse testis (B), SGC7901 (C), K562 (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD) -
Immunofluorescent analysis of GPR66 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.