说明书
MSDS产品描述：Rabbit polyclonal antibody to 4EBP1 (Phospho-T37)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T37 of human 4EBP1 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/100 - 1/200)基因名称：EIF4EBP1相关名称：Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
基因编号(人)：
1978;
基因编号(小鼠)：
13685;
基因编号(大鼠)：
116636;
蛋白编号(人)：
Q13541;
蛋白编号(小鼠)：
Q60876;
蛋白编号(大鼠)：
Q62622;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of 4EBP1 (Phospho-T37) expression in HEK293T insulin-treated (A), NIH3T3 insulin-treated (B), PC12 insulin-treated (C) whole cell lysates. (Predicted band size: 12 kD; Observed band size: 15 kD) -
Immunohistochemical analysis of 4EBP1 (Phospho-T37) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.