产品描述：Rat monoclonal antibody APC labled to IL-4免疫原：CHO-expressed recombinant human IL-4纯化方式：The antibody was purified by affinity chromatography.克隆类型：Monoclonal标记物：APC产品形式：Rat IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.稀释比：10 μl / assay基因名称：IL4相关名称：Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra 基因编号(人)： 3565; 蛋白编号(人)： P05112; 使用指导：1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).储存效期：Shipped and store at 4°C for one year. Do not freeze.