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CFA8517
产品名称:Anti-CD326 Antibody-Biotin labled
货号:CFA8517
来源:Mouse
反应物种:H
实验应用:IF, FC
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
100 Assays
4000
25 Assays
1600
Ship in 3 days
产品描述:Mouse monoclonal antibody Biotin labled to CD326
免疫原:Native purified human CD326.
纯化方式:The antibody was purified by affinity chromatography.
克隆类型:Monoclonal
标记物:Biotin
产品形式:Mouse IgG1. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
稀释比:10 μl / assay
基因名称:EPCAM
相关名称:GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD antigen CD326
基因编号(人): 4072;
蛋白编号(人): P16422;
使用指导:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
储存效期:Shipped and store at 4°C for one year. Do not freeze.
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